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Sample preparation
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Gravimetric dilutors
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Lab blenders
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NEWDispensing pumps
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Lab accessories
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Bacterial analysis
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Automatic platers
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NEWColony counters and inhibition zone readers
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Real-time incubator and colony counter
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NEWPetri dish labeling solution
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Consumables
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Blender bags with filter
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Blender bags without filter
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Blender bags with culture media
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Sterile sampling bags
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Autoclave deodorants
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Comparison of manual and automatic methods in microbiology
The use of an automated method for the analysis of samples increases not only reproducibility but also productivity and reduces the use of consumable up to 75%. It brings repeatability and complete traceability.
Manual method
Chronometer 16:40
Total
analysis time
Automatic method
Chronometer 5:30
Total
analysis time
75%
less
consumables
1. Dilute the sample
OK
Automatic method
Gravimetric dilutors
Automatic dilution with DiluFlow
Automatically dilute the sample with the appropriate mass of diluent.
Chronometer 1:05
- Fast: 225 mL in 8 s
- Dilution accuracy: > 99% (in Standard or Accurate mode under optimal conditions)
- Reproducible
- Secure
- Automatic traceability
No
Manual method
Manual dilution
Weight and dilute a the sample
Weigh the sample and manually add the diluent.
Chronometer 4:00
- Long and tedious
- Lack of precision
- Not reproducible
- Lack of security
- Manual traceability
2. Blend the sample
OK
Manual method
Laboratory blenders
Blending the sample with BagMixer
Chronometer 2:00
- Time saving on cleaning
- Filtration and blending
- No cross-contamination
- Optimal bacterial extraction
No
Manual method
Sample grinding
Sample grinding in a blender
Chronometer 3:00
- Time consuming cleaning
- No filtration
- Risk of cross-contamination
- Undesirable cell lysis
3. Collect the filtrate
OK
Automatic method
Filter bags for blenders
Collect the filtrate in an interscience filter bag
- Instant filtration
- Easy pipetting
No
Manual method
Bag without filter
Collect the filtrate in a bag without filter
- No filtration
- Blocked pipette
4. Dilute and plate the sample
OK
Automatic method
Automatic platers
Automatic plating with easySpiral
Chronometer 0:25
- From 100 to 1x107 CFU/mL on 1 Petri dish
- Up to 75% savings in consumables
- Automatic traceability to automatic colony counters with dataLink
75% savings on consumables automatic method
No
Manual method
Manual plating
Manual serial dilutions
Chronometer 3:40
- Repetitive action method: 4 dilutions + 4 successive platings
- Lots of consumables
- Manual traceability
Consumables used with the manual method
5. Counting the colonies
OK
Automatic method
Automatic colony counters
Simplified counting with filter bag - Correlation for automatic counting
Chronometer 2:00
- Counting is simplified with the use of a filter bag
- Standardized and reproducible results (Study conducted on Bacillus cereus, Escherichia coli and Lactobacillus casei.)
- Results exported and archived automatically
Traceability export data - Connection SIL/LIMS
Connexion LIMS
Traceability export data - 21 CFR Part 11
Audit trail
Bar-code
Bar-code
Import data traceability - DataLink
dataLink
Traceability export data - PDF
Traceability export data - Saving and archiving sessions
Backup and archive sessions
Traceability export data - JPG
JPG, PNG, BMP
Traceability export data - CSV
CSV
Traceability export data
ExcelTM OpenOfficeTM
No
Manual method
Manual counting
Counting is difficult with a bag without filter - Correlation for manual counting
Chronometer 6:00
- Difficult counting with the use of a bag without a filter
- Variable and uncertain results
- Results entered manually:
error-prone and time-consuming
